monitoring virus harvesting time in embryonated chicken eggs inoculated with avian influenza h9n2 vaccine strain
نویسندگان
چکیده
knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. the present study aimed to evaluate the best harvesting time of h9n2 avian influenza virus (aiv) vaccine strain inoculated in specific pathogen free (spf) embryonated chicken eggs (ece)s. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated into 336, 11-day old eces at the rate of 0.1 ml/ece via intera-allantoic. amnio-allantoic fluid (aaf) and chorioalantoic membranes were collected at 2 hours intervals up to 96 post inoculations from 7 eggs in each trial. the presence of virus in the harvested suspensions was evaluated by hemagglutination assay (ha) and egg infective dose 50 (eid50) assays. our results showed that, the best virus harvesting time for vaccine production is between 50 – 60 hours post inoculation (hpi) in eces. this finding may provide possibility of the sufficient and increasing rate of production, immunogenicity and economic factors in h9n2 influenza vaccine production.
منابع مشابه
Monitoring virus harvesting time in embryonated chicken eggs inoculated with avian influenza H9N2 vaccine strain
Knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. The present study aimed to evaluate the best harvesting time of H9N2 avian influenza virus (AIV) vaccine strain inoculated in specific pathogen free (SPF) embryonated chicken eggs (ECE)s. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated into 336, 11-day old ECE...
متن کاملOptimization of incubation temperature in embryonated chicken eggs inoculated with H9N2 vaccinal subtype of avian influenza virus
There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into ...
متن کاملEvolution of H9N2 avian influenza virus in embryonated chicken eggs with or without homologous vaccine antibodies
BACKGROUND Vaccines constitute a unique selective pressure, different from natural selection, drives the evolution of influenza virus. In this study, A/Chicken/Shanghai/F/1998 (H9N2) was continually passaged in specific pathogen-free embryonated chicken eggs with or without selective pressures from antibodies induced by homologous maternal antibodies. Genetic mutations, antigenic drift, replica...
متن کاملOptimization of incubation temperature in embryonated chicken eggs inoculated with H9N2 vaccinal subtype of avian influenza virus
There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10(-5) dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into 400, 11-d...
متن کاملoptimization of incubation temperature in embryonated chicken eggs inoculated with h9n2 vaccinal subtype of avian influenza virus
there are little information about growth properties of low pathogenic (lp) avian influenza virus (aiv) in embryonated chicken eggs (eces) at different incubation temperatures. knowledge of this information increases the quantity and quality of antigen in vaccine production process. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated (intra-allantoic) into 400, 11-day...
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عنوان ژورنال:
archives of razi instituteناشر: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
دوره 69
شماره 1 2016
کلمات کلیدی
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